SKU: 16-0023
{"url":"https://www.epicypher.com/products/nucleosomes/variant-nucleosomes-vnucs/mononucleosome-h3-1-n2-recombinant-human-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":692,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"","category":["Nucleosomes/Variant Nucleosomes (vNucs)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/692/761/nuc_image__44909.1606315336.png?c=2","alt":"Mononucleosome (H3.1ΔN2), Recombinant Human, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=692","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"583","name":"Pack Size","value":"50 µg"}],"sku":"16-0023","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Mononucleosome (H3.1ΔN2), Recombinant Human, Biotinylated Description:</b> Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A,\nH2B, H3.1ΔN2 and H4; accession numbers: H2A-P04908; H2BO60814; H3.1-P68431; H4-P62805) with the amino acid sequence of H3 beginning with threonine 3, wrapped by 147 base pairs of 601 positioning sequence DNA. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has\nhigh affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Mononucleosome (H3.1ΔN2), Recombinant Human, Biotinylated</b></span></span> Formulation:</b> Mononucleosome (H3.1ΔN2), Recombinant Human, Biotinylated (27.3 μg protein weight, 50 μg DNA+protein) in μl 10 mM Tris HCl,\npH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 199,405.26 Da.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Mononucleosome (H3.1ΔN2), Recombinant Human, Biotinylated Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For\nbest results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Mononucleosome (H3.1ΔN2), Recombinant Human, Biotinylated Application Notes:</b> Mononucleosome (H3.1ΔN2), Recombinant Human, Biotinylated are highly purified and are suitable for use as substrates in enzyme screening assays, structural studies, or for effector protein binding experiments. The N-terminal deletion allows for the study of the role of the N-terminus in many aspects of chromatin biology.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b> Lowary PT and J Widom (1998). J Mol Biol 276: 19-42.\nLuger K et al (1999). Methods Mol Biol 119: 1-16.<br /> <br /></span></span><br /></p></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0023.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-0023 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr></tbody></table></td><td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0023-detail-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0023-detail-data.jpg\" alt=\"16-0023 Reader Data\" width=\"125\" height=\"325\" /></a> </td></tr><tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Detailed Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in WT Mononucleosomes (Lane 1) resolved alongside proteins in Mononucleosome (H3.1ΔN2), Human Recombinant Biotinylated (Lane 2). The faster migration of the H3.1ΔN2 observed in Lane 2 as compared to Lane 1 denotes the deletion of the amino acids from the N-terminus of the histone protein. <br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0023-MS-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0023-MS-data.jpg\" alt=\"16-0023 MS Data\" width=\"125\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong>H3.1ΔN2 protein analyzed by high resolution mass spectrometry. Expected mass = 15,045.6 Da. Determined mass = 15,045.5 Da.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0023-protein-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0023-protein-gel.jpg\" alt=\"16-0023 Protein Gel Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in\nMononucleosome (H3.1ΔN2), Recombinant Human, Biotinylated (1 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3.1ΔN2, and H4) are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0023-DNA-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0023-DNA-gel.jpg\" alt=\"16-0023 DNA Gel Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Mononucleosome (H3.1ΔN2), Recombinant Human, Biotinylated run on a native PAGE gel and stained with ethidium bromide to visualize DNA. <strong>Lane 1:</strong> Free DNA (100 ng). <strong>Lane 2:</strong> Intact nucleosomes (300 ng).<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> </td></tr></tbody></table>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$475.00","value":475,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":84,"sku":"16-0006","name":"Mononucleosomes, Recombinant Human Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-recombinant-human-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Recombinant Nucleosomes (rNucs)"],"summary":"\n \n \n \n Description\n \n \n Mononucleosomes ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/84/331/Human_recombinant_nucleosome__14471.1516390310.gif?c=2","alt":"Mononucleosomes, Recombinant Human 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Pack Size: 50 µg
